topB [2017-05-09 15:36:35]

DNA topoisomerase III

locus: BSU04260

pI: 9.88

mw: 81.29 kDa

protein length: 727 aa

gene length: 2181 bp

function: replication

product: DNA topoisomerase III

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]

Gene

Coordinates: 476,558 → 478,741

The protein

Catalyzed reaction/ biological activity

• ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)

Protein family

• prokaryotic type I/III topoisomerase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|TopA]

[SW|Domains]

• N-terminal [SW|TOPRIM domain] (aa 3-136) [Pubmed|9722641]

Structure

• [PDB|2GAI] (from ''Thermotoga maritima'', 26% identity) [Pubmed|16600296]

Expression and Regulation

Operons

• genes: [gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]

  description

  • [pubmed|22383849]

• genes: [gene|AC2618C53DA020F2CD5192AEE462D15E8EE0F57F|lrpC]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]

  description

  • [pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|10913073], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-C111 (topB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2109 NBRP B. subtilis, Japan]
• BKE04260 (''topB''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab)
• GP1864 (''topB''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
• GP1896 (''topB''::''cat'') (available in [SW|Jörg Stülke]'s lab)

References

• 17114254,16600296,9722641